Journal of Nanomaterials & Molecular NanotechnologyISSN: 2324-8777

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Research Article, J Nanomater Mol Nanotechnol Vol: 2 Issue: 5

Fibroblast Behavior on PMMAEA and PMMAEA-Collagen Films and Nanofibers

Wen Hu1, Jon Holy2 and Xun Yu1*
1Department of Mechanical and Energy Engineering, University of North Texas, TX 76203, USA
2Department of Anatomy, Microbiology and Pathology, University of Minnesota School of Medicine-Duluth, Duluth, MN 55812, USA
Corresponding author : Dr. Xun Yu
Department of Mechanical and Energy Engineering, University of North Texas, TX 76203, USA
Tel: 940-565-2742; Fax: 940-369-8675
E-mail: [email protected]
Received: July 10, 2013 Accepted: September 18, 2013 Published: September 23, 2013
Citation: Hu W, Holy J, Yu X (2013) Fibroblast Behavior on PMMAEA and PMMAEA-Collagen Films and Nanofibers. J Nanomater Mol Nanotechnol 2:5. doi:10.4172/2324-8777.1000122

Abstract

Fibroblast Behavior on PMMAEA and PMMAEA-Collagen Films and Nanofibers

The influence of different physical forms of substrates on fibroblast behavior was examined by comparing the following experimental and control groups: 1) glass coverslips, 2) poly(methyl methacrylateco- ethyl acrylate) (PMMAEA) cast films, 3) electrospun PMMAEA nanofibers, 4) electrospun PMMAEA/collagen nanofibers, and 5) electrospun collagen. Cell adhesion, spreading and proliferation were compared on the different substrates. It was observed that fibroblasts on electrospun PMMAEA, PMMAEA-collagen, and collagen substrates spread more slowly after plating, and did not spread out to the extent observed for glass or PMMAEA films. Cells on electrospun fibers exhibited more filopodial-like structures and fewer stress fibers than the glass and PMMAEA film surfaces. Cell viability studies showed that although cells remained viable on all substrates, proliferation was faster on glass and PMMAEA films than on electrospun substrates. Overall, fibroblast behavior appeared to more closely resemble in vivo behavior on the electrospun nanofibers than on films or glass substrates.

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