Journal of Nanomaterials & Molecular NanotechnologyISSN: 2324-8777

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Research Article, J Nanomater Mol Nanotechnol Vol: 5 Issue: 6

An Investigation of In vitro Cytotoxicity of Silver Nanoparticles (Ag-NPs) on Cancerous Cell Lines

Gowramma B, Dharaneeswara Reddy D and Muralidhara Rao D*
Department of Biotechnology, Sri Krishnadevaraya University, Anantapuram, Andhra Pradesh, India
Corresponding author : Muralidhara Rao D
Department of Biotechnology, Sri Krishnadevaraya University, Anantapuram, Andhra Pradesh, India
Tel: +919940699873
Fax: 08554 255244, 08554 255805
E-mail: [email protected]
Received: May 03, 2016 Accepted: November 15, 2016 Published: November 20, 2016
Citation: Gowramma B, Dharaneeswara Reddy D, Muralidhara Rao D (2016) An Investigation of In vitro Cytotoxicity of Silver Nanoparticles (Ag-NPs) on Cancerous Cell Lines. J Nanomater Mol Nanotechnol 5:6. doi: 10.4172/2324-8777.1000204

Abstract

An Investigation of In vitro Cytotoxicity of Silver Nanoparticles (Ag-NPs) on Cancerous Cell Lines

Cytotoxicity and apoptotic effect of silver nanoparticles (Ag-NPs) was estimated in cancerous Hepatocellular carcinoma (HepG2), Human breast cancer (MCF-7), Human neuroblastoma (SK-N-SH) and Rat glioma (C6) cell lines, which were produced from Corynebacterium glutamicum. The studies of cytotoxicity were carried out under normoxia and hypoxia conditions. The cell lines of Ag-NPs cytotoxicity were demonstrated by MTT assay and half maximum inhibitory concentrations (IC50) were evaluated. Ag-NPs were exhibited good cytotoxic effects in C6 followed by HepG2, MCF-7 and SK-N-SH. Reactive Oxygen Species (ROS) production in all cell lines was examined. Analysis of apoptosis and necrosis was carried out by annexin-V/ PI staining method using flow cytometry. The present study was designed to recognize the anticancer property of Ag-NPs against HepG2, MCF-7, C6 and SK-N-SH and to the best of our knowledge this is the first report on cytotoxic activity of Ag-NPs synthesized from Corynebacterium glutamicum.

Keywords: Corynebacterium; Silver nanoparticles; Cytotoxicity; Apoptosis; IC50; MTT; ROS; Cancerours cell lines

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