Journal of Nanomaterials & Molecular NanotechnologyISSN: 2324-8777

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Research Article, J Nanomater Mol Nanotechnol Vol: 5 Issue: 4

Biofabrication of Antibacterial and Antioxidant Silver Nanoparticles (Agnps) by an Endophytic Fungus Pestalotia Sp. Isolated from Madhuca Longifolia

Satish K Verma*, Surendra K Gond, Ashish Mishra, Vijay K Sharma, Jitendra Kumar, Dheeraj K Singh and Ravindra N Kharwar*
International School of Photonics, Cochin University of Science and Technology, Cochin, Kerala, India
Corresponding authors: Ravindra N Kharwar
Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, India
Tel: 91-542-6701095
E-mail: [email protected]
Satish K Verma
Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, India
Tel: 91-542-6701095
E-mail: [email protected]
Received: April 07, 2016 Accepted: May 27, 2016 Published: June 02, 2016
Citation: Verma SK, Gond SK, Mishra A, Sharma VK, Kumar J, et al. (2016) Biofabrication of Antibacterial and Antioxidant Silver Nanoparticles (Agnps) by an Endophytic Fungus Pestalotia Sp. Isolated from Madhuca Longifolia. J Nanomater Mol Nanotechnol 5:3. doi:10.4172/2324-8777.1000189

Abstract

Biofabrication of Antibacterial and Antioxidant Silver Nanoparticles (Agnps) by an Endophytic Fungus Pestalotia Sp. Isolated from Madhuca Longifolia

Particles of metals or other materials in nanometre size are called as nanoparticles (NPs). Of ten endophytic fungi screened, seven endophytic fungi namely Alternaria alternata, Botryodiplodia sp., Pestalotia sp., Fusarium sp., Aspergillus sp. Aschersonia sp. and Phomopsis sp. were able to synthesize silver nanoparticles. Silver metal nanopartites were synthesised successfully by both mycelium as well as cell filtrate of Pestalotia sp., which was initially observed by spectrometric analysis. TEM pictures revealed the synthesis of angular shape of silver nanoparticles like trigonal, pentagonal, hexagonal, few of cuboids and some were of spherical in shape too. Most of nanoparticles (76%) were found below the size of 40 nm. FTIR spectra peaks showed the presence of -NH, CO, C=C and C-H containing organic metabolites which might play a role in stabilizing the nanoparticles. Silver nanoparticles solution (0.5 mM) inhibited the growth significantly than control against all pathogenic bacteria tested. Mycosynthesized AgNPs were most active against Aeromonas hydrophila @2 μg/ml IC50 followed by Staphylococcus aureus (6 μg/ml), Escherichia coli (8μg/ml), Morganella morganii (24 μg/ml), Salmonella typhi (42 μg/ml), Enterococcus faecalis (40 μg/ml) and Klebsiella pneumoniae (50 μg/ml) respectively. Silver nanoparticles had also shown antioxidant activity with DPPH free radical scavenging with inhibitory concentration (IC50) at 47 μg /ml. To combat with new emerging pathogenic bacteria that threat our society by causing dreadful diseases, we require the development of effective therapeutics. Thus, eco-friendly synthesised antimicrobial and antioxidant silver nanoparticles allow us to suitably employing their application in nanoparticles mediated drug delivery and therapy of diseases.

Keywords: Nanoparticles; Antibacterial; Antioxidant; Endophytic fungi; Cell filtrate

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