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Journal of Nanomaterials & Molecular NanotechnologyISSN: 2324-8777

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Research Article, J Nanomater Mol Nanotechnol Vol: 7 Issue: 6

Structural Characterisation and Bactericidal activity of Biogenic AgNPs using Cladosporium cladosporioides

Bhat MA1,2*

1Department of Biotechnology, Government College for Women, Nawakadal Srinagar, India

2Department of Biomedical Engineering, Sathyabama University, Chennai, India

*Corresponding Author : M Amin Bhat
Department of Biotechnology, Government College for Women, Nawakadal Srinagar, India,
Tel: +91 9858355943
E-mail: [email protected]

Received: August 01, 2018 Accepted: September 10, 2018 Published: September 15, 2018

Citation: Bhat MA (2018) Structural Characterisation and Bactericidal activity of Biogenic AgNPs using Cladosporium cladosporioides. J Nanomater Mol Nanotechnol 7:6. doi: 10.4172/2324-8777.1000254

Abstract

The plant pathogenic fungus, Cladosporium cladosporioides was used for the preparation of metallic silver nanoparticles. The HRTEM analysis confirms the formation of silver nanoparticles with having an average size of 27 ± 5 nm. FTIR spectroscopic analysis was carried out to identify the functional groups involved in the reducing/capping of silver ions into silver nanoparticles. The AFM analysis confirmed that AgNPs were uniform in size with average roughness 17.7638 nm and stability in size through line profiling. The AgNPs were investigated for their antibiotic efficacy against clinical pathogens and found remarkable positive results. The MIC was found to be 20 μM to 25 μM/ml for AgNPs. The present study concluded that the silver nanoparticles could be used as better drugs and may potentially eliminate the higher doses of antibiotics due to their vital role inhibiting bacterial growth as compared to the common antibiotics.

Keywords: Cladosporium cladosporioides; UV-Vis Spectrophotometer; FTIR; AFM; HR-TEM; MIC; Antibacterial activity

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