Journal of Food and Nutritional DisordersISSN: 2324-9323

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Research Article, J Food Nutr Disor Vol: 2 Issue: 2

Effective Concentration Method for the Recovery of Crude Alkaline Phosphatase fromthe Clarified Hepatopancreatic Tissue Homogenate of Shrimp by Ammonium Sulfate Precipitation

Krishna Prasad Nooralabettu* and Kathyayani Puttige
Department of Biotechnology, P.A. College of Engineering, Nadupadavu, Mangalore-574153, Karnataka, India
Corresponding author : Krishna Prasad Nooralabettu
Department of Biotechnology, P.A. College of Engineering, Nadupadavu, Mangalore-574153, Karnataka, India
Tel: +919448529048
E-mail: [email protected]
Received: January 14, 2013 Accepted: May 22, 2013 Published: May 27, 2013
Citation: Krishna Prasad N, Kathyayani P (2013) Effective Concentration Method for the Recovery of Crude Alkaline Phosphatase from the Clarified Hepatopancreatic Tissue Homogenate of Shrimp by Ammonium Sulfate Precipitation. J Food Nutr Disor 2:2. doi:10.4172/2324-9323.1000111

Abstract

Effective Concentration Method for the Recovery of Crude Alkaline Phosphatase fromthe Clarified Hepatopancreatic Tissue Homogenate of Shrimp by Ammonium Sulfate Precipitation

Hepatopancreas of shrimps is an important organ functions in food absorption, transport, secretion of digestive enzymes, and storage of lipids, glycogen, and a number of minerals. Hence, impurities present in the hepatopancreas are the bottle neck during the purification of alkaline phosphatase to commercially exploit it, including its use as replacement enzyme in the gastrointestinal tract. Concentration of the alkaline phosphatase by exploiting physico-chemical properties of the components of hepatopancreatic tissue homogenate by ammonium sulfate precipitation is critical at the initial stages of enzyme purification itself as it efficiently reduces the bulk of the volume and partially purifies the enzyme.

Keywords: Alkaline phosphatase; Shrimps; Insoluble; Centrifugation; Clarification

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