Journal of Food and Nutritional DisordersISSN: 2324-9323

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Research Article, J Food Nutr Disor Vol: 5 Issue: 5

Development of a High- Performance Liquid Chromatography with Diode- Array Detection Method using Monolithic Column for Simultaneous Determination of Five Tetracyclines Residues in Fish Muscles

Ibtissem Ghorbel-Abid1,2*, Habibi Belhassen2,3, Rim Lahsini3, Dalila Chehimi Ben Hassen2 and Malika Trabelsi-Ayadi2
1Laboratory of Materials, Processing and Analysis (LMTA). National Institute of Research and Physical and Chemical Analysis (INRAP) BiotechPole, Sidi Thabet 2032, Ariana Tunisia
2Laboratory for Applications of Chemistry to Resources, Natural Substances and Environment-(LACReSNE). Carthage University, Faculty of Sciences of Bizerte, Zarzouna 7021, Bizerte, Tunisia.
3Laboratory of Research and Development, (SIPHAT), Ben Arous 2036,Tunisia
Corresponding author : Ibtissem Ghorbel-Abid
Institute National Research and Physical and Chemical Analysis (INRAP) Laboratory Materials Processing and Analysis (LMTA), Thabet BiotechPoleSidi-2032-Ariana, Tunisia
Tel: +216 21 024 843
Fax: +216 72 590 566
E-mail: [email protected] mail.com
Received: July 01, 2016 Accepted: August 16, 2016 Published: August 22, 2016
Citation: Ghorbel-Abid I, Belhassen H, Lahsini R, Ben Hassen DC, Trabelsi-Ayadi M (2016) Development of a High-Performance Liquid Chromatography with Diode-Array Detection Method using Monolithic Column for Simultaneous Determination of Five Tetracyclines Residues in Fish Muscles. J Food Nutr Disor 5:5. doi:10.4172/2324-9323.1000209

Abstract

Development of a High- Performance Liquid Chromatography with Diode- Array Detection Method using Monolithic Column for Simultaneous Determination of Five Tetracyclines Residues in Fish Muscles

A simple and accurate high performance liquid chromatography with diode array detection method was developed and validated for simultaneous determination of residues of five tetracyclines in fish muscles. Chromatographic separation was achieved in 14 min using a monolithic column, monitoring was at 355 nm and the mobile phase was consisting of oxalic acid, acetonitrile and methanol. Samples were extracted and purified using solid phase extraction cartridge with mean recoveries ranged from 72.1% to 92.2%. The current method was validated and applied on fish muscle in aquaculture according to the commission decision criteria 2002/657/EC. Samples spiked at different concentration levels (0.5, 1 and 1.5 times the maximum residue limit) were analyzed. It is demonstrated that the new method is robust for detection and quantitative determination of tetracyclines residues. Decision limit (CCα) ranged from 117 to 128 μg Kg-1 and capacity of detection (CCβ) from 118 to 154 μg Kg-1.

Keywords: Aquaculture; Tetracyclines; MRL; HPLC-DAD; SPE

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