Journal of Food and Nutritional Disorders ISSN: 2324-9323

Research Article, J Food Nutr Disord Vol: 6 Issue: 1

Serotypes, Virulence and Antibiotic Susceptibility of Salmonella Spp. Strains, Isolated from Poultry Meat Cutting Parts in Greater Tunis (Tunisia)

Walid Oueslati1*, Mohamed Ridha Rjeibi2, Abdelfettah Ettriqui1 and Samia Zrelli1
1Laboratory of Food Microbiology, University Manouba, National School of Veterinary Medicine of Sidi Thabet, 2020 Sidi Thabet, Tunisia
2Laboratory of Parasitology, University Manouba, National School of Veterinary Medicine of Sidi Thabet, 2020 Sidi Thabet, Tunisia
Corresponding author : Walid Oueslati
Laboratory of Food Microbiology, University Manouba, National School of Veterinary Medicine of Sidi Thabet, 2020 Sidi Thabet, Tunisia
Tel:
71 552 470
E-mail: [email protected]
Received: September 07, 2016 Accepted: January 18, 2017 Published: January 23, 2017
Citation: Oueslati W, Rjeibi MR, Ettriqui A, Zrelli S (2017) Serotypes, Virulence and Antibiotic Susceptibility of Salmonella Spp. Strains, Isolated from Poultry Meat Cutting Parts in Greater Tunis (Tunisia). J Food Nutr Disor 6:1. doi: 10.4172/2324-9323.1000217

Abstract

Serotypes, Virulence and Antibiotic Susceptibility of Salmonella Spp. Strains, Isolated from Poultry Meat Cutting Parts in Greater Tunis (Tunisia)

This study was conducted to estimate the infection rate, antibiotic susceptibility and serotype distribution and virulence genes of Salmonella in poultry meat cutting parts in Greater Tunis, Tunisia. In four years (2012-2015), 433 samples were sent to Food Microbiology Laboratory of the National School of Veterinary Medicine of Sidi thabet. The poultry meat cutting parts contamination prevalence by Salmonella spp. was 6.7% (29/433). The 29 isolates were positive to PCR using Salmonella specific primers (Figure 1). This rate varies from 3.1% (7/226) for skinless poultry meat cutting parts to 10.6% (22/207) for skin poultry meat cutting parts (p<0.001). A total number of 7 serotypes were identified, namely S. Kentucky (9/29), S. Anatum (7/29), S. Zanzibar (6/29), S. Newport (3/29), S. Minnesota (2/29), S. Amsterdam (1/29) and S. Corvallis (1/29) (p<0.05) (Table 1). Salmonella strains (29) were positive for invasion gene invA and negative for the virulence genes spvC and h-li (Table 1, Figure 1). All of the strains were resistant to at least one of the antibiotics. Multiresistance concerned 17/29 of the strains, including Amoxicillin (10/29), Tetracycline (8/29), Gentamicin (6/29), and Kanamycin (4/29). All S. Kentucky strains were resistant to Ciprofloxacin. Furthermore, all strains were sensitive to the association (Amoxicillin + Clavulanic acid), Cefoxitin and Ceftazidime (Table 1).

Keywords: Salmonella; Poultry meat; Serotype; Antibiotics resistance; invA; spvC; h-li; Tunisia

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