Journal of Nanomaterials & Molecular Nanotechnology
Editor-in-chief: Dennis W. Smith Jr., PhD
University of Texas at Dallas, USA
The Journal of Nanomaterials & Molecular Nanotechnology (JNMN) promotes rigorous research that makes a significant contribution in advancing knowledge for Nanomaterials and Molecular nanotechnology. JNMN includes all major themes pertaining to Nanotechnology.
Journal of Nanomaterials & Molecular Nanotechnology is a subscription based journal that provides a range of options to purchase our articles and also permits unlimited Internet Access to complete Journal content. It accepts research, review papers, online letters to the editors & brief comments on previously published articles or other relevant findings in SciTechnol. Articles submitted by authors are evaluated by a group of peer review experts in the field and ensures that the published articles are of high quality, reflect solid scholarship in their fields, and that the information they contain is accurate and reliable.
A Novel Biosensor for Detection of Salmonella typhimurium Carrying SSeC Gene Based on the Secondary Quenching Effect of Carbon Nanotubes
In this paper, a sensitive and selective biosensor was constructed for detection of Salmonella typhimurium carrying SSeC gene, based on covalently coupling of molecular beacons (MBs) stained with daunorubicins (DNR) to single-walled carbon nanotubes (SWNTs) through EDC/Sulfo-NHS chemistry. In the absence of target, the fluorescence of daunorubicin was fairly week as result of dual fluorescence quenching. On the contrary, the daunorubicin was competed from the beacon due to the target-induced formation of rigid structure between the loop structure of the MB and the target sequence, which resulted in a decrease in the effect of dual fluorescence quenching, thereby the fluorescence intensity increased substantially. The target quantum was achieved by fluorescence increment. The experimental results showed that the recovery of fluorescence of daunorubicin is proportion to theconcentration of the target DNA with the range 0.2-0.7 μM and the low detection limit is 50 nM. The fluorescence intensity did not augment considerably when other Salmonella sps. were detected via the same method, which clearly displayed a high selectivity and specificity for the biosensor. Additionally, the real samples were also detected and their low detection limits were up to 105 CFU/mL. Consequently, the biosensor should be a potential alternative to the conventional detection ones and has great prospect in pathogenic microorganisms’ detection, clinical diagnosis and treatment.
Production of Hydroxyapatite Nanoparticles Using Tricalcium- Phosphate by Alkanindiges illinoisensis
The general purpose of this study was to examine the role of urease and phosphatase in production of hydroxyapatite (HA) nanocrystal that was performed using two different media of precipitating and Pikovskaya (PVK), respectively. We evaluated properties of HA by means of X-ray diffraction (XRD) analysis and scanning electron microscopy (SEM) an equipped with energy dispersive X-ray spectroscopy (EDX). Among bacterial strains isolated from nature, only two strains C17 and C21 had the ability to produce HA nanocrystal. These strains were phosphatase-positive and produced HA only in the presence of tricalcium-phosphate as an insoluble mineral phosphorus, therefore phosphatase have a direct role in the HA production. In the other hand, none of strains which were urease-positive had any ability to produce HA, therefore urease has no role in HA production. Crystallite particle size was determined from XRD data using Scherrer formula that was sub- 25-nm. Based on data obtained, the strain C17 selected as the best HA-producing strain. The BLAST analysis showed that partial 16S rRNA sequence of this isolate is more than 99% identical of Alkanindiges illinoisensis that was deposited in GenBank with accession number JX666243. The aim of this study was to find easier and cheaper ways for HA production using tricalcium-phosphate as an inorganic phosphate instead of glycerol-2- phosphate (G-2-P) that was used by previous researchers.
Fibroblast Behavior on PMMAEA and PMMAEA-Collagen Films and Nanofibers
The influence of different physical forms of substrates on fibroblast behavior was examined by comparing the following experimental and control groups: 1) glass coverslips, 2) poly(methyl methacrylateco- ethyl acrylate) (PMMAEA) cast films, 3) electrospun PMMAEA nanofibers, 4) electrospun PMMAEA/collagen nanofibers, and 5) electrospun collagen. Cell adhesion, spreading and proliferation were compared on the different substrates. It was observed that fibroblasts on electrospun PMMAEA, PMMAEA-collagen, and collagen substrates spread more slowly after plating, and did not spread out to the extent observed for glass or PMMAEA films. Cells on electrospun fibers exhibited more filopodial-like structures and fewer stress fibers than the glass and PMMAEA film surfaces. Cell viability studies showed that although cells remained viable on all substrates, proliferation was faster on glass and PMMAEA films than on electrospun substrates. Overall, fibroblast behavior appeared to more closely resemble in vivo behavior on the electrospun nanofibers than on films or glass substrates.
Effects of the Nanostructured Complex of Biologically Active Compounds on the Free-Radical Processes and the Liver State of the Chicken Cross "Shaver 2000"
Double handling of hatching eggs by nanostructured complex of the biologically active compounds (ethanolamine, succinic acid and serine) significantly influenced embryogenesis of poultry. This handling has a positive influence on the state of their liver. These results can be explained by decreasing degree of lipid peroxidation and increasing capacity of the “antioxidant defense” system. Peroxidase activity decreased by 20.8% and superoxide dismutase increased by 1.7 times. The proposed approach leads to an increase in the viability of experimental chickens and acceleration of embryogenesis.