Journal of Pharmaceutical Sciences & Emerging Drugs ISSN: 2380-9477

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Research Article, J Pharm Sci Emerg Drugs Vol: 2 Issue: 1

Separation and Identification of Swertiamarin from Enicostema axillare Lam.Raynal by Centrifugal Partition Chromatography and Nuclear Magnetic Resonance-Mass Spectrometry*

Virendra S. Rana*
Indian Agricultural Research Institute (Indian Council of Agricultural Research), Pusa, New Delhi, India
Corresponding author : Virendra S.Rana
Indian Agricultural Research Institute (Indian Council of Agricultural Research), Pusa, New Delhi, India
Tel: 011-25841390
E-mail: [email protected]
Received: July 12, 2014 Accepted: September 06, 2014 Published: October 15, 2014
Citation: Rana VS. (2014) Separation and Identification of Swertiamarin from Enicostema axillare Lam. Raynal by Centrifugal Partition Chromatography and Nuclear Magnetic Resonance-Mass Spectrometry*. J Pharm Sci Emerg Drugs 2:1. doi:10.4172/2380-9477.1000102

Abstract

Separation and Identification of Swertiamarin from Enicostema axillare Lam.Raynal by Centrifugal Partition Chromatography and Nuclear Magnetic Resonance-Mass Spectrometry*

Enicostema axillare, a bitter herb, has been used for the treatment of diabetes in traditional system of medicine in India. Swertiamarin, a bitter and potent hepatoprotective iridoid glucoside (~20 mg) was isolated by Centrifugal Partition Chromatography coupled with Diode Array Detector (DAD) from the crude methanolic extract (1.0 g) of its aerial parts using a biphasic solvent system composed of chloroformmethanol- water (86:74:20, v/v) in ascending mode for the first time. The purity of the compound was found to be >97%. The structure of swertiamarin was determined using spectroscopic data mainly NMR (1H, 13C, DEPT, HSQC and HMBC) and ESI-MS. Compound was also identified by comparing 13C NMR data with those reported and some of the 13C NMR values were revised.

Keywords: Gentianaceae; Enicostema axillare; Swertiamarin; CPC; NMR; ESI

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